| Unlock This | How to Unlock |
| King Boo, Petey Piranha, Piranha Pipes (Petey Piranha's Kart) |
Finish the Star Cup in Mirror Mode |
| Tilt-A-Kart (Battle Mode Arena) |
Finish the Flower Cup in Mirror Mode |
| Toadette Kart (Toadette's Kart) |
Finish the Mushroom Cup in Mirror Mode |
| Mirror Mode (Selectable at the CC selection) |
Finish the All Cup Tour in 150CC |
| All Cup Tour (All 4 Cups in one) |
Finish the Special Cup in 150CC |
| Barrel Train (Diddy Kong's Kart) |
Finish the Star Cup in 150CC |
| Turbo Birdo (Birdo's Kart) |
Finish the Flower Cup in 150CC |
| Luigi's Mansion (Battle Mode Arena) |
Finish the Mushroom Cup in 150CC |
| Toad, Toadette, Toad Kart (Toad's Kart) |
Finish the Special Cup in 100CC |
| Special Cup (Wario Colosseum/Dino Dino Jungle/Bowser's Castle/Rainbow Road) |
Finish the Star Cup in 100CC |
| Waluigi Racer (Waluigi's Kart) |
Finish the Flower Cup in 100CC |
| Rattle Buggy (Baby Luigi's Kart) |
Finish the Mushroom Cup in 100CC |
| Bullet Blaster (Bowser Jr.'s Kart) |
Finish the Special Cup in 50CC |
| Para Wing (Koopa Paratroopa's Kart) |
Finish the Star Cup in 50CC |
| Bloom Coach (Daisy's Kart) |
Finish the Flower Cup in 50CC |
| Green Fire (Luigi's Kart) |
Finish the Mushroom Cup in 50CC |
| Boo Pipes (King Boo's Kart) |
Finish the Special Cup in Mirror Mode |
| Parade Kart (Belongs to Toadsworth, but he doesn't race) |
Finish the All Cup Tour in Mirror Mode |
| Alternative Start Screen |
Earn Gold trophies for all cups in all race classes |
| Alternative "Thanks for Playing" Screen |
Earn Gold trophies for all cups in all race classes |
| Peety Pirahnah |
Beat all cups |
| Boo |
Beat all cups |
