| Unlock This | How to Unlock |
| Sound Check Mode |
Beat every layout on every skill level with each character |
| Red Blade Mode |
Get a Grandmaster or Master of Assassins Ranking in all 18 levels |
| Ninja Rebirth Item |
Complete Training Stage |
| Rin's hidden costume, Ayame's Tenchu 3 costume |
Beat the game on any difficulty. |
| Ayame's Tenchu 1 and Tenchu 2 costumes |
Defeat Juzo in Pit Fight mode with more than 23 minutes remaining and defeat the two Ayames. Alternatively, collect a total of 1600 scrolls. |
| Pit Fight mode |
Beat the game on Normal difficulty. |
| Pit Fight mode Boss Fights |
Defeat all 6 enemy groups in Pit Fight mode. |
| Unlock This | How to Unlock |
| 2.1 Departures |
Do not Eliminate all enemies in mission 1 |
| 2.2 Determinationkill |
Eliminate all enemies in mission 1 |
| 6.1 The Blind Masseuse |
At least one enemy spot you in mission 5 |
| 6.2 What Lurks in the shadow |
You may not be spotted in mission 5 |
| 7.1 A Sister's Promise |
Pick up the map in mission 6 |
| 7.2 For Whom the Bell Tolls |
Dont Pick up the map in mission 6 |
| 10.1 Flames of Passion |
Dont Eliminate the samurai at the beginning of kilmission 9 |
| 10.2 Flames of Sorrow |
Eliminate all 3 enemies at the beginning of mission 9 |
| 12.1 Tenchu Kurenai |
Eliminate Ranzo |
| 12.2 Tenchu Kurenai |
Let Ranzo escape |
